NME3 Antilichaam/nucleosidedifosfaat kinase 3

Western Blot: 0.25-0.5 µg/mL

Q13232

Human, Mouse, Rat

A synthetic peptide corresponding to a sequence in the middle region of human NME3 was used as the immunogen for the NME3 antibody.

WB

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This NME3 antibody is available for research use only.

After reconstitution, the NME3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the NME3 antibody should be determined by the researcher.

Western blot analysis of NME3 using anti-NME3 antibody. Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human 293T whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NME3 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. A predominant band is detected at an approximately 17 kDa in human cell lines, slightly below the predicted ~19 kDa size and consistent with the processed mature form of NME3. SiHa and THP-1 lysates also show a weaker band near 18 kDa, likely representing a partially processed or differently modified NME3 species. Rat kidney displays a band close to 19 kDa, consistent with species specific differences in processing or migration.