NOLC1 Antilichaam / Nucleolaire en opgerolde lichaamsfosfoproteïne 1 / NOPP140

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200, Immunocytochemistry/Immunofluorescence: 1:50-1:200

Q14978

Human

A synthesized peptide derived from human NOLC1 was used as the immunogen for the NOLC1 antibody.

30N31

WB, IHC, ICC, IF

Affinity chromatography

Liquid

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

This NOLC1 antibody is available for research use only.

Store the NOLC1 antibody at -20°C.

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Optimal dilution of the NOLC1 antibody should be determined by the researcher.

Western blot analysis of NOLC1 using anti-NOLC1 antibody. Lane 1: human Hela whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOLC1 antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. NOLC1 western blot across human cell lines shows a predominant band at ~130 kDa. Although the predicted mass is ~74 kDa, NOLC1/Nopp140 is highly acidic and heavily phosphorylated, causing it to migrate markedly above its theoretical size on SDS-PAGE.