Normale controlenaam IgG2 (HRP)

Llama IgG2 isotype control (which consists of IgG2a, IgG2b and IgG2c isotypes) has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa. No bands corresponding to llama IgG1 or IgG3 are observed.

Control Antibodies

1 mg/mL

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide

Room Temp

Store vial at 4˚ C prior to restoration. For extended storage aliquot contents and freeze at -20˚ C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4˚ C as an undiluted liquid. Dilute only prior to immediate use. Restore with 100 uL deionized water (or equivalent)

493.6 . 96.1

IgG2

Llama IgG2 HRP conjugated isotype control can be utilized in Flow Cytometry, ELISA, and western blotting experiments where determination of sample isotype is important.

ELISA, WB, Flow

Lyophilized