NOS2 Antilichaam / iNOS

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.

Western blot: 1:1000-1:2000, Immunohistochemistry (FFPE) : 1:25-1:50, Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25

P35228

Human

A portion of amino acids 830-860 from the human protein were used as the immunogen for the NOS2 antibody.

WB, IHC-P, FACS, IF

Antigen affinity purified

Purified

This NOS2 antibody is available for research use only.

Aliquot the NOS2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

In 1X PBS, pH 7.4, with 0.09% sodium azide

The stated application concentrations are suggested starting points. Titration of the NOS2A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Cytoplasmic

IHC testing of FFPE human brain tissue with NOS2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.