NPT Antilichaam (44D1]

Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph (3') -II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.

11979140

Q79C30

Q79C30

Human

Ni-NTA purified truncated recombinant NPT expressed in E. Coli strain BL21 (DE3) .

4B4D1

Unconjugated

Primary Antibodies

Signal Transduction

Antibody is purified by protein A affinity chromatography.

Batch dependent

None

Blue Ice

NPT monoclonal antibody can be stored at -20˚ C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG2a

Npt

Neomycin phosphotransferase

Plasmid pRL765

Landis WG et.al Human and ecological Risk Assessment 6 (5) : 875-899.

Stewart CN et.al BioTechniques 29 (4) : 832-843.

Thomson JA et.al Journal of Food Science 66 (2) : 188-193.

ELISA, WB

81919445

Liquid