NRGN cDNA

Neurogranin (NRGN) is the human homolog of the neuron-specific rat RC3/neurogranin gene. This gene encodes a postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. The NRGN gene contains four exons and three introns. The exons 1 and 2 encode the protein and exons 3 and 4 contain untranslated sequences. It is suggested that the NRGN is a direct target for thyroid hormone in human brain, and that control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development as well as in adult subjects.

Neurogranin, NRGN, HNG, RC3

11q24

Human

PATGen (puc19-derived cloning vector)

ATGGACTGCTGCACCGAGAACGCCTGCTCCAAGCCGGACGACGACATTCTAGACATCCCGCTGGACGATCCCGGCGCCAACGCGGCCGCCGCCAAAATCCAGGCGAGTTTTCGGGGCCACATGGCGCGGAAGAAGATAAAGAGCGGAGAGCGCGGCCGGAAGGGCCCGGGCCCTGGGGGGCCTGGCGGAGCTGGGGTGGCCCGGGGAGGCGCGGGCGGCGGCCCCAGCGGAGACTAG

Neurogranin, NRGN, HNG, RC3, ATGD0219-10 µg, ATGD0219-20 µg, ATGD0219-50 µg, ATGD0219-100 µg, ATGD0219-250 µg, ATGD0219-500 µg, ATGD0219-1 mg, ATGD0219-010, ATGD0219-020, ATGD0219-050, ATGD0219-100, ATGD0219-250, ATGD0219-500, ATGD0219-01M

Store the plasmid at -20C.

Lyophilized

Neuroscience

602350

NP_001119653.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_001126181.1

237bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.