NRXN2 Antilichaam / Neurexine 2

Western Blot: 0.25-0.5 µg/mL

P58401

Mouse, Rat

A synthetic peptide corresponding to a sequence at the N-terminus of human NRXN2 was used as the immunogen for the NRXN2 antibody.

WB

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This NRXN2 antibody is available for research use only.

After reconstitution, the NRXN2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the NRXN2 antibody should be determined by the researcher.

Western blot analysis of NRXN2 using anti-NRXN2 antibody. Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NRXN2 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. A diffuse ~185 kDa band is detected in rat and mouse brain lysates, consistent with the heavily glycosylated alpha isoform of NRXN2. The hazy migration pattern reflects heterogeneous glycosylation and alternative splicing commonly reported for neurexins in neuronal tissue.