NSDHL Antilichaam / NADP-afhankelijk steroïde dehydrogenase-achtig eiwit

Western blot: 1:500-1:2000, Immunoprecipitation: 1:50

Q15738

Human, Mouse, Rat

A synthesized peptide derived from human NSDHL was used as the immunogen for the NSDHL antibody.

30N66

WB, IP

Affinity chromatography

Liquid

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

This NSDHL antibody is available for research use only.

Store the NSDHL antibody at -20°C.

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Optimal dilution of the NSDHL antibody should be determined by the researcher.

Western blot analysis of NSDHL using anti-NSDHL antibody. Lane 1: human whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSDHL antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. NSDHL antibody detects a single band migrating at ~38 kDa across the indicated cell and tissue lysates. Although the theoretical molecular weight is ~42 kDa, NSDHL is an ER-associated membrane protein that typically migrates faster on SDS-PAGE due to signal peptide cleavage and altered SDS binding. The observed size is consistent with published reports of the mature NSDHL form.