NSMAF Antilichaam / Neutrale sphingomyelinase activeringsfactor

Western Blot: 0.25-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

Q92636

Human, Mouse, Rat

E.coli-derived human NSMAF recombinant protein (Position: K51-L868) was used as the immunogen for the NSMAF antibody.

WB, FACS, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This NSMAF antibody is available for research use only.

After reconstitution, the NSMAF antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the NSMAF antibody should be determined by the researcher.

Flow Cytometry analysis of HEL cells using anti-NSMAF antibody. Overlay histogram showing HEL cells stained with (Blue line) . The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NSMAF antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.