Numb Antilichaam

Protein numb homolog is a protein that in humans is encoded by the NUMB gene. It is mapped to 14q24.2 to q24.3. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The primary function of Numb in cell differentiation is as an inhibitor of Notch signaling which is essential for maintaining self-renewal potential in stem and progenitor cells. Numb also plays a crucial role in asymmetrical cell division during development, allowing for differential cell fate specification in the central and peripheral nervous systems. What�s more, the numb gene protein product controls binary cell fate decisions in the peripheral and central nervous systems of both invertebrates and mammals during neurogenesis.

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

P49757

Human, Monkey, Mouse, Rat

An E.coli-derived human recombinant protein (amino acids E59-A567) was used as the immunogen for the NUMB antibody.

WB, FACS, ELISA

Antigen affinity purified

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This NUMB antibody is available for research use only.

After reconstitution, the NUMB antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the NUMB antibody should be determined by the researcher.

Flow cytometry testing of fixed and permeabilized human RT4 cells with NUMB antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= NUMB antibody.