NUP62 Antilichaam / Nucleoporin 62

ELISA: 0.1-0.5 µg/mL, Immunofluorescence: 5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Western Blot: 0.25-0.5 µg/mL, Flow cytometry: 1-3ug/million cells (Intracellular)

P37198

Human, Mouse

E.coli-derived human NUP62 recombinant protein (Position: E404-D522) was used as the immunogen for the NUP62 antibody.

ELISA, IF, IHC, ICC, WB, FACS (Intracellular)

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This NUP62 antibody is available for research use only.

After reconstitution, the NUP62 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the NUP62 antibody should be determined by the researcher.

Nuclear, cytoplasmic

Immunofluorescent staining of NUP62 using anti-NUP62 antibody (red) . NUP62 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-NUP62 antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.