NXT1 cDNA

NXT1 encoded by this gene is located in the nuclear envelope. NXT1 has protein similarity to nuclear transport factor 2. NXT1 functions as a nuclear export factor in both RAN (Ras-related nuclear protein) - and CRM1 (required for chromosome region maintenance) -dependent pathways. It is found to stimulate the export of U1 snRNA in RAN- and CRM1-dependent pathways and the export of tRNA and mRNA in a CRM1-independent pathway. The encoded protein heterodimerizes with Tap protein and may regulate the ability of Tap protein to mediate nuclear mRNA export. The use of alternate polyadenylation sites has been found for NXT1.

MTR2, P15

20p12-p11.2

Human

PATGen (puc19-derived cloning vector)

ATGGCATCTGTGGATTTCAAGACCTATGTGGATCAGGCCTGCAGAGCTGCTGAGGAGTTTGTCAATGTCTACTACACCACCATGGATAAGCGGCGGCGTTTGCTGTCCCGCCTGTACATGGGCACAGCCACCCTGGTCTGGAATGGCAATGCTGTTTCAGGACAAGAATCCTTGAGTGAGTTTTTTGAAATGTTGCCTTCCAGCGAGTTCCAAATCAGCGTGGTAGACTGCCAGCCTGTTCATGATGAAGCCACACCAAGCCAGACCACGGTCCTTGTTGTCATCTGTGGATCAGTGAAGTTTGAGGGGAACAAACAACGGGACTTCAACCAGAACTTCATCCTGACCGCCCAGGCCTCACCCAGCAACACAGTGTGGAAGATCGCAAGTGACTGCTTCCGCTTCCAGGACTGGGCCAGCTAG

NXT1, MTR2, P15, NXT1, ATGD0151-10 µg, ATGD0151-20 µg, ATGD0151-50 µg, ATGD0151-100 µg, ATGD0151-250 µg, ATGD0151-500 µg, ATGD0151-1 mg, ATGD0151-10, ATGD0151-20, ATGD0151-50, ATGD0151-100, ATGD0151-250, ATGD0151-500, ATGD0151-1

Store the plasmid at -20C.

Lyophilized

Signal Transduction

605811

NP_037380.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_013248.2

423bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.