OIFN tau Recombinant eiwit

Source : Escherichia Coli. Interferon-Tau Ovine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 172 amino acids and having a molecular mass of 19914.7 Dalton.The IFNau is purified by proprietary chromatographic techniques. IFNau is also known as TP-1 (trophoblast protein-1) is a new class of type I IFN that is secreted by the trophoblast and is the signal for maternal recognition of pregnancy in sheep. IFNtau has potent immunosuppressive and antiviral activities similar to other type I IFN but is less cytotoxic than IFNlpha and IFNeta. The current investigation concerns the effect of recombinant ovine IFNtau (rOIFNtau) on the modulation of MHC class I and II expression on cloned mouse cerebrovascular endothelial (CVE) cells. IFNau induced tyrosine phosphorylation of Stat1 and upregulated the expression of MHC class I on CVE. One proposed action by which type I IFN reduces the relapse rate in MS is via interference with IFN-induced MHC class II expression. IFNtau was shown to downregulate IFN-induced MHC class II expression on CVE and, hence, may be of potential therapeutic value in downregulating inflammation in the central nervous system (CNS) . IFNtau did not upregulate the expression of MHC class II on CVE. IFNtau also inhibited the replication of Theiler's virus in CVE.

IFN-tau1||Trophoblast protein 1||TP-1||Trophoblastin||Antiluteolysin||Trophoblast antiluteolytic protein||IFN-tau||Interferon tau-1.

Greater than 95.0% as determined by both: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Lyophilized from (1mg/mL) solution containing PBS pH-7.4.

Lyophilized Interferon-Tau although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-Tau should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.

It is recommended to reconstitute the lyophilized Interferon Tau in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 10,000,000IU/mg.

The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Tyr-Leu-Ser-Arg.