OMI Antilichaam / HTRA2

Serine protease HTRA2, mitochondrial, also called Omi stress-regulated endoprotease (OMI), is an enzyme that in humans is encoded by the HTRA2 gene. This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

O43464

Human

An E.coli-derived human recombinant protein (amino acids R30-E458) was used as the immunogen for the OMI antibody.

WB, IHC-P, IF, FACS, ELISA

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This OMI antibody is available for research use only.

After reconstitution, the OMI Antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the OMI antibody should be determined by the researcher.

Cytoplasm (Mitochondria, ER)

IHC staining of FFPE human liver cancer tissue with OMI antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.