ORAI3 Antilichaam [22G9]

ORAI3 Monoclonal Antibody: Antigen stimulation of immune cells triggers Ca++ entry t hrough Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2. Larger molecular weight bands are sometimes seen in SDS-PAGE; these may represent post-translationally modified ORAI 3.

93129

Q9BRQ5

Q9BRQ5

Human, Rat

A 19 amino acid synthetic peptide from near the carboxy terminus of human ORAI3.

2H2G9

Unconjugated

Primary Antibodies

Immunology

ORAI3 Monoclonal Antibody is immunoaffinity chromotography purified IgG.

Cat. No. 1466 - Rat Spleen Tissue Lysate

1 mg/mL

ORAI3 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

ORAI3 monoclonal antibody can be stored at -20˚ C, stable for one year.

IgG2a

ORAI3

ORAI calcium release-activated calcium modulator 3

Homo sapiens

Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521.

Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.

Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins - a new paradigm in inter-organelle communication. Biochim. Biophys. Acta. 2006; 1763:1161-8.

Mercer JC, DeHaven WI, Smyth JT, et al. Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1. J. Biol. Chem. 2006; 281:24979-90.

ORAI3 Antibody [2H2G9] : TMEM142C

ELISA, WB, IHC-P, IF

74732916

Liquid