ORC1 Antilichaam

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

4998

Q13415

Human

Recombinant fusion protein containing a sequence corresponding to amino acids 696-856 of human ORC1 (NP_001177748.1) .

Unconjugated

Primary Antibodies

Cell Cycle

Affinity purification

HeLa

Batch dependent

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

None

Blue Ice

Store at -20˚ C. Avoid freeze / thaw cycles.

Observed: 90kDa

IgG

WB: 1:500 - 1:2000

ORC1

293T

A-431

K-562

Jurkat

MCF7

Origin recognition complex subunit 1

Homo sapiens

HSORC1L, PARC1, ORC1, origin recognition complex subunit 1, origin recognition complex, subunit 1 homolog, replication control protein 1

WB

Liquid