Ornithine transcarbamylase Antilichaam / OTCase / OTC

Ornithine transcarbamylase is an enzyme that is responsible for catalyzing the reaction between ornithine and carbamoyl phosphate, ultimately forming citrulline. Citrulline then goes on to participate in the urea cycle, where it helps convert toxic ammonia into urea, which can be safely excreted by the body. A deficiency in this enzyme can lead to a rare genetic disorder known as ornithine transcarbamylase deficiency. Individuals with this condition are unable to properly metabolize ammonia, leading to a buildup of toxic levels in the blood. This can result in a range of symptoms, from mild to severe, including fatigue, seizures, and even coma if left untreated.

Western blot: 1:1000-1:2000, Immunohistochemistry (FFPE) : 1:10-1:50, Flow cytometry: 1:10-1:50 per million cells in 0.1ml

P00480

Human, Mouse, Rat

A portion of amino acids 71-98 from the human protein was used as the immunogen for the Ornithine transcarbamylase antibody.

WB, IHC-P, FACS

Antigen affinity

Purified

This Ornithine transcarbamylase antibody is available for research use only.

Aliquot the Ornithine transcarbamylase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

In 1X PBS, pH 7.4, with 0.09% sodium azide

The stated application concentrations are suggested starting points. Titration of the Ornithine transcarbamylase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

IHC staining of FFPE human hepatocarcinoma tissue with Ornithine transcarbamylase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.