Osm Antilichaam / Oncstatine

Western Blot: 0.25-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL

Q65Z15

Mouse, Rat

E.coli-derived rat Osm recombinant protein (Position: E55-D158) was used as the immunogen for the Osm antibody.

WB, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This Osm antibody is available for research use only.

After reconstitution, the Osm antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the Osm antibody should be determined by the researcher.

Western blot analysis of Osm using anti-Osm antibody. Lane 1: rat testis tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse tetis tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Osm antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A predominant band is detected at an approximately 22 kDa in all samples, consistent with the C-terminally processed mature form of OSM, which has been reported to migrate between ~22 and 28 kDa depending on proteolytic processing and glycosylation.