Osteoprotegerine/TNFRSF11B-eiwit (Zijn label)

Osteoprotegerin or TNFRSF11B is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. Osteoprotegerin/TNFRSF11B acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. This protein may inhibit the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. Osteoprotegerin/TNFRSF11B also play a role in preventing arterial calcification, act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis.

OCIF Protein; OPG Protein; PDB5 Protein; TR1 Protein

NP_002537.3

Human

Human

His-Tag

See Datasheet send with the product

< 1.0 EU per μg protein as determined by the LAL method.

> 95 % as determined by SDS-PAGE.

Immobilized Osteoprotegerin Protein, Human at 2 μg/mL (100 μL/well) can bind RANKL Protein, Human. The EC50 is 13-64 ng/mL.

391

Lyophilized

PBS, pH 7.4

See Datasheet

55 kDa (glycosylated)

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

HEK 293 cells