OTUB1 Antilichaam / Ubiquitinthioesterase OTUB1

Ubiquitin thioesterase OTUB1 also known as otubain-1 is an enzyme that in humans is encoded by the OTUB1 gene. The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants.

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

Q96FW1

Human, Mouse, Rat

An E.coli-derived human recombinant protein (amino acids K10-K271) was used as the immunogen for the OTUB1 antibody.

WB, IHC-P, FACS, ELISA

Antigen affinity purified

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This OTUB1 antibody is available for research use only.

After reconstitution, the OTUB1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the OTUB1 antibody should be determined by the researcher.

IHC staining of FFPE human rectum adenocarcinoma tissue with OTUB1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.