OTUD3 Antilichaam / OTU domein-bevattend eiwit 3

Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, ELISA: 0.1-0.5 µg/mL

Q5T2D3

Human

E.coli-derived human OTUD3 recombinant protein (Position: E51-I398) was used as the immunogen for the OTUD3 antibody.

WB, ICC, IF, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This OTUD3 antibody is available for research use only.

After reconstitution, the OTUD3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the OTUD3 antibody should be determined by the researcher.

Immunofluorescent staining of OTUD3 using anti-OTUD3 antibody (red) . OTUD3 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-OTUD3 antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.