P16 Antilichaam [2D9A12]

The progression of cells through the cell cycle is regulated by a family of protein kinases known as cyclin-dependent kinases (Cdks) . The sequential activation of individual members of this family and their consequent phosphorylation of critical substrates promotes orderly progression through the cell cycle. The cyclins function as differentially expressed positive regulators of Cdks. Negative regulators of the cycle include the p53-inducible 21 kDa WAF1/Cip1 protein designated p21, Kip1 p27 and p16. The complexes formed by Cdk4 and the D-type cyclins have been strongly implicated in the control of cell proliferation during the G1 phase. It has recently been shown that p16 binds to Cdk4 and inhibits the catalytic activity of the Cdk4/cyclin D complex. Moreover, the gene encoding p16 exhibits a high frequency of homozygous deletions and point mutations in established human tumor cell lines.

1029

P42771

NP_000068.1

Human, Rat

Ni-NTA purified truncated recombinant P16 expressed in E. Coli strain BL21 (DE3) .

2D9A12

Unconjugated

Primary Antibodies

Other

Batch dependent

Ascitic fluid containing 0.03% sodium azide.

None

Blue Ice

P16 (Mouse and Human) monoclonal antibody can be stored at -20˚ C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG2b

CDKN2A

Cyclin-dependent kinase inhibitor 2A

Homo sapiens

Hunter, T. 1993. Cell 75: 839-841.

Sherr, C.J. 1993. Cell 73: 1059-1065.

El-Deiry, W.S., et al. 1993. Cell 75: 817-825.

ARF, MLM, P14, P16, P19, CMM2, INK4, MTS1, TP16, CDK4I, CDKN2, INK4A, MTS-1, P14ARF, P19ARF, P16INK4, P16INK4A, P16-INK4A

ELISA, WB, IHC

4502749