P16-INK4a-TAT Recombinant eiwit

P16-INK4a is a nuclear protein that regulates the cell cycle by inhibiting cyclin dependent kinase-4 (CDK4) and CDK6. p16-INK4a inhibits CDK activity by binding to the CDK molecules in a manner that interferes with their ability to interact with cyclin D. This activity has the effect of suppressing tumor formation and growth, and of inducing replicative senescence in various normal cells, including stem cells. The expression of p16-INK4a steadily increases with age and tends to accumulate in stem cell compartments. The deletion, rearrangement, or mutation of the p16-INK4a gene is frequently found in melanomas as well as in certain other types of cancer. P16-INK4a and other transcription factors have been introduced into cells by DNA transfection, viral infection, or microinjection. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors and other nuclear proteins into primary as well as transformed cells. Recombinant human p16-INK4a-TAT expressed in E. coli is a 18 kDa protein containing 167 amino-acid residues, including the 156 residues of full-length p16-INK4a and a 13-residue C-terminal TAT peptide (GGYGRKKRRQRRR) .

1029

P42771

NP_000068.1

Recombinant Proteins

E. coli

Cancer, Homeostasis, Signal Transduction

Greater than or equal to 95% by SDS-PAGE gel and HPLC analyses.

Room Temp

The lyophilized p16-INK4a-TAT recombinant protein is stable for at least 2 years from date of receipt at -20˚ C. Reconstituted p16-INK4a-TAT is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚ C. As with any protein, exposing p16-INK4a-TAT recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Cyclin-dependent kinase inhibitor 2A

Homo sapiens

ARF, MLM, P14, P16, P19, CMM2, INK4, MTS1, TP16, CDK4I, CDKN2, INK4A, MTS-1, P14ARF, P19ARF, P16INK4, P16INK4A, P16-INK4A, Cyclin-dependent kinase 4 inhibitor A

Human

4502749

Lyophilized