P4HA1 Antilichaam / Prolyl 4-hydroxylase subunit alpha 1

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunofluorescence: 5 µg/mL

P13674

Human

A synthetic peptide corresponding to a sequence in the middle region of human P4HA1 was used as the immunogen for the P4HA1 antibody.

WB, IHC, IF

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This P4HA1 antibody is available for research use only.

After reconstitution, the P4HA1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the P4HA1 antibody should be determined by the researcher.

Cytoplasm

Immunofluorescent staining of P4HA1 using anti-P4HA1 antibody (red) . P4HA1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/ml rabbit anti-P4HA1 antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.