P53 (fosfo Ser46) Antilichaam

P53 is a nuclear protein which plays an essential role in the regulation of cell cycle specifically in the transition from G0 to G1. It is found in very low levels in normal cells however in a variety of transformed cell lines in high amounts and believed to contribute to transformation and malignancy. The open reading frame of p53 is 393 amino acids long, with the central region (consisting of amino acids from about 100 to 300) containing the DNA-binding domain. This proteolysis-resistant core is flanked by a C-terminal end mediating oligomerization and an N-terminal end containing a strong transcription activation signal. p53 binds as a tetramer to a PBS (p53-Binding Site) and activates the expression of downstream genes that inhibit growth and/or invasion. p53 binds as a tetramer to a p53-binding site (PBS) and to activate the expression of adjacent genes that inhibit growth and/or invasion. Deletion of one or both p53 alleles reduces the expression of tetramers, resulting in decreased expression of the growth inhibitory genes

7157

P04637

NP_000537.3

Human

P53 (Phospho-Ser46) antibody was raised against a peptide sequence around phosphorylation site of serine 46 (M-L-S (p) -P-D) derived from Human p53.

Unconjugated

Primary Antibodies

Phospho-Specific, Apoptosis, Cancer, Cell Cycle

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Phospho-Specific

Blue Ice

Store antibody at -20˚ C for up to one year.

53 kDa

This antibody detects endogenous level of p53 only when phosphorylated at serine 46.

Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200

TP53

Tumor protein p53

Homo sapiens

Dhavan, R. and Tsai, L. H. (2001) Nat Rev Mol Cell Biol. 2: 749-759. Patrick, G. N. et al. (1998) J Biol Chem. 273: 24057-24064. Di Stefano V, et al. (2005) Oncogene. 24 (35) : 5431-5442. Mayo LD, et al. (2005) J Biol Chem. 280 (28) : 25953-25959. Wang L, et al. (2005) Oncogene. 24 (18) : 3020-3027.

P53, BCC7, LFS1, TRP53, ERBB, ERBB1, HER1, Tumor suppressor p53, Phosphoprotein p53, Antigen NY-CO-13, TP53

WB, IF

120407068

Liquid