PAF-AH

Platelet Activating Factor (PAF) is a biologically active phospholipid, which exerts primarily proinflammatory activities by specifically signaling through G-protein-coupled receptors on platelets, neutrophils, and monocytes. Platelet Activating Factor Acetylhydrolase (PAF-AH) is a secreted protein that mediates PAF activity by specifically catalyzing hydrolysis of the “sn2” ester bond, resulting in the conversion of PAF to the biologically inactive lyso-PAF. PAF-AH can also interact with LDL particles to induce the hydrolysis of LDL associated, oxidized phospholipids, generating lysophosphatidylcholine (lyso-PC) and other lysophospholipids. Recombinant PAF-AH is a 420 amino acid glycoprotein which migrates with an apparent molecular mass of 47-55 kDa by SDS-PAGE analysis.

PAF-acetylhydrolase, PAF 2-acylhydrolase, LDL-PLA82), 2-acetyl-1-alkylglycerophosphacholine esterase

5049

P68402

NP_002563.1

NM_002572.3

11q23

Human

Human

FDWQYINPVA HMKSSAWVNK IQVLMAAASF GQTKIPRGNG PYSVGCTDLM FDHTNKGTFL RLYYPSQDND RLDTLWIPNK EYFWGLSKFL GTHWLMGNIL RLLFGSMTTP ANWNSPLRPG EKYPLVVFSH GLGAFRTLYS AIGIDLASHG FIVAAVEHRD RSASATYYFK DQSAAEIGDK SWLYLRTLKQ EEETHIRNEQ VRQRAKECSQ ALSLILDIDH GKPVKNALDL KFDMEQLKDS IDREKIAVIG HSFGGATVIQ TLSEDQRFRC GIALDAWMFP LGDEVYSRIP QPLFFINSEY FQYPANIIKM KKCYSPDKER KMITIRGSVH QNFADFTFAT GKIIGHMLKL KGDIDSNVAI DLSNKASLAF LQKHLGLHKD FDQWDCLIEG DDENLIPGTN INTTNQHIML QNSSGIEKYN

< 0.1 ng/µg of protein (< 1EU/µg)

> 95% by SDS-PAGE & HPLC analyses

Measured by its ability to cleave a PAF analog in a chromogenic substrate linked assay. At a PAF-AH concentration of 10.0 μg/ml, 50% cleavage was achieved at an incubation time of approximately 2 minutes.

420

Lyophilized

47-55 kDa

HEK 293 cells