PAFAH1B1 Antilichaam / Lissencephaly 1 / LIS1

Platelet-activating factor acetylhydrolase IB subunit alpha, also called Lissencephaly 1 and LIS1, is an enzyme that in humans is encoded by the PAFAH1B1 gene. This locus was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. This gene encodes the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase, a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine) . Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits, the other, a single subunit. In addition, a single-subunit isoform of this enzyme is found in serum.

Western blot: 0.5-1 µg/mL, ELISA: 0.1-0.5 µg/mL

P43034

Human

An E.coli-derived human recombinant protein (amino acids I95-R410) was used as the immunogen for the PAFAH1B1 antibody.

WB, ELISA

Antigen affinity purified

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This PAFAH1B1 antibody is available for research use only.

After reconstitution, the PAFAH1B1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the PAFAH1B1 antibody should be determined by the researcher.

Western blot testing of human U-87 MG cell lysate with PAFAH1B1 antibody. Predicted molecular weight ~47 kDa.