PAG1 Antilichaam / Cbp / Fosfoproteïne geassocieerd met glycosfingolipide verrijkt microdomeinen 1

Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry/Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

Q9NWQ8

Human, Mouse, Rat

A synthetic peptide corresponding to a sequence at the N-terminus of human PAG1 was used as the immunogen for the PAG1 antibody.

WB, ICC/IF, FACS

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This PAG1 antibody is available for research use only.

After reconstitution, the PAG1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the PAG1 antibody should be determined by the researcher.

Cytoplasm, plasma membrane

Immunofluorescent staining of PAG1 using anti-PAG1 antibody (green) . PAG1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-PAG1 antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.