PC-3 Lysaat

PC3 cells are human prostate cancer cell lines that from a bone metastasis of a grade IV prostatic adenocarcinoma from a 62 year old male. These cells are useful in investigating the biochemical changes in advanced prostatic cancer cells and in assessing their response to chemotherapeutic agents. Moreover, they can be used to create subcutaneous tumors in mice in order to investigate a model of the tumor environment in the context of the organism.

Human

WB

2 mg/mL (determined by BCA assay)

PC3, ATGL0004-10 µg, ATGL0004-20 µg, ATGL0004-50 µg, ATGL0004-100 µg, ATGL0004-250 µg, ATGL0004-500 µg, ATGL0004-1 mg, ATGL0004-10, ATGL0004-20, ATGL0004-50, ATGL0004-100, ATGL0004-250, ATGL0004-500, ATGL0004-1

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Lysates

Human

All lanes : PC-3 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196)

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody