PCCA Antibody / Propionyl-CoA carboxylase alfa

The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Multiple transcript variants encoding different isoforms have been found for this gene.

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

P05165

Human, Mouse, Rat

An E.coli-derived human recombinant protein (amino acids E403-E728) was used as the immunogen for the PCCA antibody.

WB, IHC-P, FACS, ELISA

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This PCCA antibody is available for research use only.

After reconstitution, the PCCA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the PCCA antibody should be determined by the researcher.

Cytoplasm

IHC staining of FFPE human liver cancer tissue with PCCA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.