PCDHGA2 Antilichaam / Protocadherine gamma A2

This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.

Western blot: 1-2 µg/mL, ELISA: 0.1-0.5 µg/mL

Q9Y5H1

Human, Mouse

An E.coli-derived human recombinant protein (amino acids R132-D444) was used as the immunogen for the PCDHGA2 antibody.

WB, ELISA

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This PCDHGA2 antibody is available for research use only.

After reconstitution, the PCDHGA2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the PCDHGA2 antibody should be determined by the researcher.

Western blot testing of 1) human MCF7, 2) human A549 and 3) mouse lung tissue lysate with PCDHGA2 antibody. Predicted molecular weight ~101 kDa and ~90 kDa (two isoforms) .