PCDHGC4 Antilichaam / Protocadherine gamma-C4

This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.

Western blot: 1-2 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

Q9Y5F7

Human, Rat

An E.coli-derived human recombinant protein (amino acids D57-H770) was used as the immunogen for the PCDHGC4 antibody.

WB, IF, FACS, ELISA

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This PCDHGC4 antibody is available for research use only.

After reconstitution, the PCDHGC4 Antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the PCDHGC4 antibody should be determined by the researcher.

Immunofluorescent staining of FFPE human U-2 OS cells with PCDHGC4 antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.