PCMT1 Antilichaam/eiwit-L-isoaspartaat (D-aspartaat) O-methyltransferase

Protein-L-isoaspartate (D-aspartate) O-methyltransferase is an enzyme that in humans is encoded by the PCMT1 gene. This gene encodes a member of the type II class of protein carboxyl methyltransferase enzymes. The encoded enzyme plays a role in protein repair by recognizing and converting D-aspartyl and L-isoaspartyl residues resulting from spontaneous deamidation back to the normal L-aspartyl form. The encoded protein may play a protective role in the pathogenesis of Alzheimer's disease, and single nucleotide polymorphisms in this gene have been associated with spina bifida and premature ovarian failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

P22061

Human, Mouse, Rat

An E.coli-derived human recombinant protein (amino acids M1-K227) was used as the immunogen for the PCMT1 antibody.

WB, IHC-P, IF, FACS, ELISA

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This PCMT1 antibody is available for research use only.

After reconstitution, the PCMT1 Antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the PCMT1 antibody should be determined by the researcher.

Cytoplasm

Western blot testing of 1) rat testis, 2) rat brain, 3) rat liver, 4) rat PC-12, 5) mouse testis, 6) mouse brain, 7) mouse liver and 8) mouse NIH 3T3 cell lysate with PCMT1 antibody. Predicted molecular weight ~25 kDa.