PD-1 Antilichaam

PD-1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC) . T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases.

5133

Q15116

Q15116

Human, Mouse, Rat

4065

Unconjugated

Primary Antibodies

KO Validation (Figure 1) : Anti-PD-1 antibodies (4065) specificity was further verified by PD-1 specific knockout. PD-1 signal was not detected in PD-1 knockout HeLa cells as compared to that in control wild type cells. KO Validation4065) KD validation (Figure 2) : Anti-PD-1 antibody (4065) specificity was further verified by PD-1 specific knockdown. PD-1 signal in HeLa cells transfected with PD-1 siRNAs or shRNA was disrupted in comparison with that in cells transfected with control siRNAs. KD validation4065

Apoptosis

PD-1 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1204 - K562 Cell Lysate

1 mg/mL

PD-1 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

PD-1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Human PD-1 has 1 isoform (288aa, 32kD) . Mouse PD-1 has 1 isoform 288aa, 32kD) and Rat PD-1 also has one isoform (287aa, 32kD) . 4065 can detect human, mouse and rat.

IgG

Human PD-1 has 1 isoform (288aa, 32kD) . Mouse PD-1 has 1 isoform 288aa, 32kD) and Rat PD-1 also has one isoform (287aa, 32kD) . 4065 can detect human, mouse and rat.

PDCD1

Cat. No. 1205 - Jurkat Cell Lysate

Cat. No. 1208 - THP-1 Cell Lysate

Cat. No. 10-301 - Human Brain Tissue Slide

Programmed cell death 1

Homo sapiens

Holling et al. Hum. Immunol. 2004; 65:282-90.

Ishida et al. EMBOJ. 1992;11:3887-95.

Zhong et al. Int. Immunol. 2004; 16:1181-8

Nishimura et al. Immunity 1999;11:141-51

PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, PD1, Programmed cell death protein 1, Protein PD-1, PDCD1, PDCD-1

ELISA, WB, IHC-P, IF

145559515

Liquid