PD-L1 Antilichaam

PD-L1 plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, PD-L1 modulates the activation threshold of T-cells and limits T-cell effector response (1) . The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (2,3) . Through a yet unknown activating receptor, it may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (4) .

29126

Q9NZQ7

NP_054862

Human, Mouse, Rat

4059

Unconjugated

Primary Antibodies

Independent Antibody Validation (Figure 2) shows similar PD-L1 expression profile in both human and mouse cell lines detected by two independent anti-PD-L1 antibodies that recognize different epitopes, 4059 against the center of human PD-L1 and RF16035 against the extracellular domain. PD-L1 proteins are detected in most of the cell lines, but not in A549 and THP-1 cells by the two independent antibodies. Independent Antibody Validation4059 RF16035 siRNA Knockdown Validation (Figure 3) : Anti-PD-L1 antibody (4059) specificity was further verified by PD-L1 specific siRNA knockdown. PD-L1 signal in HeLa cells transfected with PD-L1 siRNAs was much weaker in comparison with that in HeLa cells transfected with control siRNAs. siRNA Knockdown Validation4059 Overexpression Validation (Figure 4) : Anti-PD-L1 antibodies (4059) can detect the overexpression of PD-L1 protein in 293 cells transfected with PD-L1. Overexpression Validation4059

Apoptosis

PD-L1 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1201 - HeLa Cell Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Rat: (77%), Mouse: (71%)

PD-L1 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

PD-L1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

PD-L1 antibody has no cross-reactivity to PD-L2.

Human PD-L1 has 3 isoforms, including isoform 1 (290aa, 33.3kD), isoform 2 (176aa, 20.2kD), and isoform 3 (178aa, 20.5kD) . This antibody detects human isoform 1&3, mouse and rat PD-L1 (290aa, 33kD for both of them) .

CD274

Cat. No. 1224 - Daudi Cell Lysate

Cat. No. 1204 - K562 Cell Lysate

Cat. No. 10-501 - Human Heart Tissue Slide

Cat. No. 1301 - Human Heart Tissue Lysate

CD274 molecule

Homo sapiens

Freeman et al. Exp. Med. 2000; 192:1027-34.

Burr et al. Nature 2017; 549:101-5.

Mezzadra et al. Nature 2017; 549:106-10.

Dong et al. Nat. Med. 1999 5:1365-9.

29416054

21523733

24955518

26483045

29024471

26351349

22461641

29704674

28751245

24866169

29034543

29610255

24130734

29309297

26175924

27799141

25672395

23606867

30059678

25937270

PD-L1 Antibody: B7-H, B7H1, PDL1, PD-L1, PDCD1L1, PDCD1LG1, Programmed cell death 1 ligand 1, B7 homolog 1

ELISA, WB, IHC-P, IF, Flow

7661534

Liquid