PD-L1 / B7-H1 / CD274 Proteïne (ECD, hFc-tag)

Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1 (PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

A530045L16Rik Protein; B7h1 Protein; Cd274 Protein; Pdcd1l1 Protein; Pdcd1lg1 Protein; Pdl1 Protein

NP_068693.1

Mouse

Mouse

HFc-Tag

See Datasheet send with the product

< 1.0 EU per μg protein as determined by the LAL method.

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

1.Measured by its binding ability in a functional ELISA. 2. Immobilized Recombinant Mouse PD-L1 / B7-H1 / CD274 Protein (ECD, Fc Tag) at 2 μg/mL (100 μL/well) can bind Recombinant Mouse PD-1 Protein, Biotinylated. The EC50 is 200-750 ng/mL. 3. Immobilized Recombinant Mouse PD-1 Protein, Biotinylated at 2 μg/mL (100 μL/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Mouse PD-L1 / B7-H1 / CD274 Protein (ECD, Fc Tag) . The EC50 is 4-12 ng/mL. 4. Loaded Recombinant Mouse PD-L1/B7-H1 Protein, hFc Tag on ProA Biosensor, can bind Recombinant Mouse PD-1 Protein, His Tag with an affinity constant of 3.6 μM as determined in BLI assay (Sartorius Octet RED384) .

459

Lyophilized or frozen liquid

PBS, pH 7.4

See Datasheet

74 kDa (glycosylated)

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

HEK 293 cells