PD-L1 / B7-H1 / CD274 Proteïne (ECD, His Tag)

Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1 (PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

A530045L16Rik Protein; B7h1 Protein; Cd274 Protein; Pdcd1l1 Protein; Pdcd1lg1 Protein; Pdl1 Protein

NP_068693.1

Mouse

Mouse

His-Tag

See Datasheet send with the product

< 1.0 EU per μg protein as determined by the LAL method.

> 98 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

1. Loaded Recombinant Mouse PD-L1/B7-H1 Protein , His Tag on HIS1K Biosensor, can bind Recombinant Mouse PD-1 Protein, hFc Tag with an affinity constant of 0.519μM as determined in BLI assay (Sartorius Octet RED384) . 2. Recombinant Mouse PD-1 Protein , hFc Tag captured on Protein A chip can bind Recombinant Mouse PD-L1/B7-H1, His Tag with an affinity constant of 2.489 µM as determined in an SPR assay (Biacore T200) . 3. Recombinant Mouse PD-L1/B7-H1 Protein, His Tag captured on CM5 chip via Anti-his antibody, can bind Recombinant Mouse PD-1 Protein, hFc Tag with an affinity constant of 1.117 µM as determined in an SPR assay (Biacore T200) .

231

Lyophilized

PBS, pH 7.4

See Datasheet

55 kDa (glycosylated)

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

HEK 293 cells