PD-L2 Antilichaam

PD-L2 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by co gnate peptides bound to MHC molecules on antigen-presenting cells (APC) . T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PD-L1 and PD-L2, both of which are thought act as a negative regulator of T cell activation. However, it has been suggested that PD-L2 can act to stimulate an immunogenic response through and alternative receptor from PD-1.

80380

Q9BQ51

NP_079515

Human, Mouse

4063

Unconjugated

Primary Antibodies

Independent Antibody Validation in Cell lines (Figure 2) shows similar PD-L2 expression profile in human and mouse cell lines detected by two independent anti-PD-L2 antibodies that recognize different epitopes, 4063 against central domain and the competitor antibody against N-terminus domain. PD-L2 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines4063 competitor antibody KO Validation (Figure 3) : Anti-PD-L2 antibodies (4063) specificity was further verified by PD-L2 specific knockout. PD-L2 signal was not detected in PD-L2 knockout HeLa cells as compared to that in control wild type cells. KO Validation4063) Recombinant Protein Test (Figure 4) : Anti-PD-L2 antibodies (4063) detected human PD-L2 recombinant protein at different concentrations. Recombinant Protein Test4063 Regulated expression validation (Figure 11) : PD-L2 expression detected by anit-PD-L2 antibodies (4063) was up-regulated by anti-PD1 antibody treatment, which was reduced by Next A alone or combination treatment (anti-PD1 antibody + NextA) . Regulated expression validation4063

Apoptosis

PD-L2 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1207 - Raji Cell Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: rat (94%) .

PD-L2 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

PD-L2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

Human PD-L2 has 3 isoforms, including isoform 1 (273aa, 31kD), isoform 2 (183aa, 21kD) and isoform 3 (182aa, 21kD) . Mouse PD-L2 has one isoform (247aa, 28kD) and Rat PD-L2 also has one isoform (268aa, 30kD) . 4063 can detect human isoform and mouse isoform.

PDCD1LG2

Cat. No. 1403 - Mouse Brain Tissue Lysate

Cat. No. 1201 - HeLa Cell Lysate

Cat. No. 1224 - Daudi Cell Lysate

Cat. No. 1282 - 3T3/NIH Cell Lysate

Programmed cell death 1 ligand 2

Homo sapiens

Holling et al. Hum. Immunol. 2004; 65:282-90.

Ishida et al. EMBO J. 1992; 11:3887-95.

LaGier and Pober. Hum. Immunol. 2006; 67:568-78.

Zhang et al. Proc. Natl. Acad. Sci. USA 2006; 103:11695-700.

25672395

30992475

PD-L2 Antibody: B7DC, Btdc, PDL2, CD273, PD-L2, PDCD1L2, bA574F11.2, B7DC, Programmed cell death 1 ligand 2, Butyrophilin B7-DC, PD-1 ligand 2

ELISA, WB, IHC-P, IF

190014605

Liquid