PD1 / PDCD1 / CD279 Proteïne (hFc-tag)

Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.

CD279 Protein; hPD-1 Protein; hPD-l Protein; hSLE1 Protein; PD-1 Protein; PD1 Protein; SLEB2 Protein

NP_005009.2

Human

Human

HFc-Tag

See Datasheet send with the product

< 1.0 EU per μg protein as determined by the LAL method.

> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.

1. Immobilized PD-1 Protein, Human (ECD, hFc Tag) at 2 μg/mL (100 μL/well) can bind PD-L2 Protein, Human, Recombinant (ECD, His Tag) . The EC50 is 45-280ng/mL. 2. Immobilized Human PD1 / PDCD1 / CD279 Protein (Fc Tag) at 2 μg/mL (100 μl/well) can bind Recombinant Human PD-L1 Protein, Biotinylated. The EC50 is 25-75 ng/mL. 3. Loaded Recombinant Human PD-1 Protein, hFc Tag on ProA Biosensor, can bind Recombinant Human PD-L2 / B7-DC Protein, His Tag with an affinity constant of 0.154 μM as determined in BLI assay (Sartorius Octet RED384) . 4. Recombinant Human PD-1 Protein, hFc Tag captured on Protein A chip, can bind Recombinant Human PD-L1/B7-H1 Protein, His Tag with an affinity constant of 1.620 μM as determined in an SPR assay (Biacore 8K) . 5. Immobilized Recombinant Human PD1 / PDCD1 / CD279 Protein (Fc Tag) at 2 μg/mL (100 μL/well) can bind Recombinant Human PD-L2 Protein, Biotinylated. The EC50 is 1.0-3.0 ng/mL. 6. Serial dilutions of Nivolumab derived Anti-PD1 Antibody were added into Recombinant Human PD1 / PDCD1 / CD279 Protein (Fc Tag) : Recombinant Human PD-L1 Protein, Biotinylated binding reactions. The half maximal inhibitory concentration (IC50) is 1.7 μg/mL. 7. Recombinant Human PD-L2/B7-DC Protein, His Tag captured on CM5 chip via Anti-His antibody, can bind Recombinant Human PD-1 Protein, hFc Tag with an affinity constant of 67.780 nM as determined in an SPR assay (Biacore T200) .

381

Lyophilized or frozen liquid

PBS, pH 7.4

See Datasheet

66 kDa

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

HEK 293 cells