PD1 / PDCD1 / CD279 Proteïne (hFc-tag)

Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.

CD279 Protein; hPD-1 Protein; hPD-l Protein; hSLE1 Protein; PD-1 Protein; PD1 Protein; SLEB2 Protein

NP_032824.1

Mouse

Mouse

HFc-Tag

See Datasheet send with the product

< 1.0 EU per μg protein as determined by the LAL method.

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ≥ 95 % as determined by SEC-MALS.

1.Immobilized Recombinant Mouse PD1 / PDCD1 / CD279 Protein (Fc Tag) at 2μg/mL (100 μL/well) can bind Recombinant Mouse PD-L1/B7-H1/CD274 Protein, Biotinylated, with a linear range of 0.16-2.5 μg/mL. 2.Recombinant Mouse PD-1 Protein , hFc Tag captured on Protein A chip can bind Recombinant Mouse PD-L1/B7-H1, His Tag with an affinity constant of 2.489 µM as determined in an SPR assay (Biacore T200) . 3.Loaded Recombinant Mouse PD-1 Protein, hFc Tag on proA Biosensor, can bind Recombinant Mouse PD-L2/B7-DC Protein, His Tag with an affinity constant of 80.700 nM as determined in BLI assay (Sartorius Octet RED384) . 4.Recombinant Mouse PD-L1/B7-H1 Protein, His Tag captured on CM5 chip via Anti-his antibody, can bind Recombinant Mouse PD-1 Protein, hFc Tag with an affinity constant of 1.117 µM as determined in an SPR assay (Biacore T200) .

381

Lyophilized

PBS, pH 7.4

See Datasheet

111 kDa (glycosylated)

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

HEK 293 cells