PDCD1-eiwit (C-human IgG1-Fc, Cynomolgus)

PDCD1

C-human IgG1-Fc

< 1.0 EU per μg protein as determined by the LAL method.

> 95 % as determined by SDS-PAGE

1. Measured by its ability to bind PD-L1 Protein, Human, Recombinant (ECD, His Tag) (Cat:10084-H08H) in a functional ELISA. 2. Immobilized Cynomolgus / Rhesus PD-L1 / B7-H1 / CD274 Protein (His Tag) (Cat:90251-C08H) at 2 μg/mL (100 μL/well) can bind PD-1 Protein, Cynomolgus, Recombinant (hFc Tag) (Cat:90311-C02H), the EC50 is 150-780 ng/mL. 3. Immobilized PD-1 Protein, Cynomolgus, Recombinant (hFc Tag) (Cat:90311-C02H) at 2 μg/mL (100 μL/well) can bind Anti-PD1 (MDX) -IgG4 Antibody (Nivolumab) (Cat:68050-H001), the EC50 is 8-50ng/mL. 4.Recombinant Cynomolgus PD-1 Protein, hFc Tag (Cat. No. 90311-C02H) captured on Protein A chip, can bind Biotinylated Recombinant Cynomolgus, Rhesus PD-L1/B7-H1 Protein, His & AVI Tag (Cat. No. 90251-C49H-B) with an affinity constant of 2.890 μM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 5.Loaded Recombinant Cynomolgus PD-1 Protein (hFc Tag) (Cat. No. 90311-C02H) on proA Biosensor, can bind Cynomolgus / Rhesus PD-L1 / B7-H1 / CD274 Protein (His Tag) (Cat. No. 90251-C08H) with an affinity constant of 5.140 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 6.Loaded Recombinant Cynomolgus PD-1 Protein (hFc Tag) (Cat. No. 90311-C02H) on proA Biosensor, can bind Recombinant Cynomolgus, Rhesus PD-L2/B7-DC Protein (His Tag) (Cat. No. 90249-K08H) with an affinity constant of 0.310 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Met1-Gln167

The recombinant cynomolgus PDCD1 consists 381 amino acids and predicts a molecular mass of 42.7 kDa.

Cynomolgus