PDI Recombinant eiwit

Source : Escherichia Coli. PDI Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 503 amino acids and having a molecular mass of 62.4 kDa. The PDI is fused to a 12 amino acid His tag (515 a.a. total) at N-terminal and purified by proprietary chromatographic techniques. Protein disulfide isomerases (PDIs) constitute a family of structurally related enzymes which catalyze disulfide bonds formation, reduction, or isomerization of newly synthesized proteins in the lumen of the endoplasmic reticulum (ER) . They act also as chaperones, and are, therefore, part of a quality-control system for the correct folding of the proteins in the same subcellular compartment. PDI has been found to have moderate effects (25-fold) on the rate of oxidative folding of proteins in vitro.Recombinant Human Protein Disulfide Isomerase is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. Recombinant PDI has been found to have moderate effects (25-fold) on the rate of oxidative folding of proteins in vitro.

Protein Disulfide Isomerase||PDI||EC 5.3.4.1||Prolyl 4-hydroxylase subunit beta||Cellular thyroid hormone-binding protein||p55||P4HB||ERBA2L||PDIA1||PO4DB||DSI||GIT||PHDB||PO4HB||PROHB||P4Hbeta.

Greater than 95.0% as determined bya) Analysis by RP-HPLC.b) Analysis by SDS-PAGE.

The PDI protein (1mg/mL) solution was lyophilized from PBS pH-7.

Lyophilized Protein Disulfide Isomerase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Human PDI should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please avoid freeze-thaw cycles.

It is recommended to reconstitute the lyophilized PDI in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Arg-Ala.