PDL1 Antilichaam [6H10]

PD-L1 plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, PD-L1 modulates the activation threshold of T-cells and limits T-cell effector response (1) . The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (2,3) . Through a yet unknown activating receptor, it may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (4) .

29126

Q9NZQ7

NP_054862

Human, Mouse

RF16035

6H10

Unconjugated

Primary Antibodies

Independent Antibody Validation (Figure 2) shows similar PD-L1 expression profile in both human and mouse cell lines detected by two independent anti-PD-L1 antibodies that recognize different epitopes, RF16035 against the center of human PD-L1 and RF16035 against the extracellular domain. PD-L1 proteins are detected in most of the cell lines, but not in A549 and THP-1 cells by the two independent antibodies. Independent Antibody ValidationRF16035 RF16035 siRNA Knockdown Validation (Figure 3) : Anti-PD-L1 antibody (RF16035) specificity was further verified by PD-L1 specific siRNA knockdown. PD-L1 signal in HeLa cells transfected with PD-L1 siRNAs was much weaker in comparison with that in HeLa cells transfected with control siRNAs. siRNA Knockdown ValidationRF16035 Overexpression Validation (Figure 1, 5, 10) : Anti-PD-L1 antibodies (RF16035) can detect the overexpression of PD-L1 protein in 293 cells transfected with PD-L1. Overexpression ValidationRF16035

Immunology

PD-L1 Antibody is supplied as protein A purified IgG1.

Cat. No. 1201 - HeLa Cell Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Rat: (77%), Mouse: (71%)

PD-L1 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

None

Blue Ice

PD-L1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG1

PD-L1 Antibody has no cross-reactivity to PD-L2.

Human PD-L1 has 3 isoforms, including isoform 1 (290aa, 33.3kD), isoform 2 (176aa, 20.2kD), and isoform 3 (178aa, 20.5kD) . This antibody detects human isoform 1&3, mouse and rat PD-L1 (290aa, 33kD for both of them) .

CD274

Cat. No. 1224 - Daudi Cell Lysate

Cat. No. 1340 - Human Stomach Carcinoma Tissue Lysate

Cat. No. 1315 - Human Tonsil Tissue

Programmed cell death 1 ligand

Homo sapiens

Freeman et al. Exp. Med. 2000; 192:1027-34.

Burr et al. Nature 2017; 549:101-5.

Mezzadra et al. Nature 2017; 549:106-10.

Dong et al. Nat. Med. 1999 5:1365-9.

PD-L1 Antibody: Programmed cell death 1 ligand-1, programmed death ligand 1, PDL1, PDL-1, B7-H1

ELISA, WB, IHC-P, ICC, IF

7661534

Liquid