Perforine-antilichaam [CB5.4]

18646

P10820

Mouse

Perforin (monoclonal CB5.4) antibody was raised against a recombinant mouse perforin, amino acids 98-534.

CB5.4

Unconjugated

Primary Antibodies

Immunology

Perforin (monoclonal CB5.4) antibody was purified to ≥95% in SDS-PAGE.

Batch dependent

PBS containing 0.02% sodium azide

None

Blue Ice

Perforin antibody can be stored at 4˚ C, stable for one year.

IgG2a

Perforin antibody recognizes only membrane bound active (non-cleaved) human FasL and not the inactive soluble FasL. This product can be used for detection of active human FasL by flow cytometry and western blot. Method: A mouse T cell clone was plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washings in PBS, 0.1% BSA, slides were incubated with 5 μ g/mL of CB5.4 antibody, washed in PBS, incubated in anti-rat IgG-HRP, washed in PBS, and developed in appropriate substrate solution (DAB or AEC) . Method: Cell extracts from the T cell clone B6.1 (2x106) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CB5.4 antibody at 1 μ g/mL. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system.

Perforin 1 (pore forming protein)

Mus musculus

The calcium-binding protein calreticulin is a major constituent of lytic granules in cytolytic T lymphocytes: M. Dupuis, et al.; J. Exp. Med. 177, 1 (1993) .

Resistance of CTL to perforin-mediated lysis. Evidence for a lymphocyte membrane protein interacting with perforin: C. Muller and J. Tschopp; J. Immunol. 153, 2470 (1994) .

Cubilin, a binding partner for galectin-3 in the murine utero-placental complex: S. Crider-Pirkle, et al.; J. Biol. Chem. 277, 15904 (2002) .

Fas-Fas Ligand Interactions Are Essential for the Binding to and Killing of Activated Macrophages by {gamma}{delta} T Cells: J.E. Dalton, et al.; J. Immunol. 173, 3660 (2004) .

WB, IHC, ICC, IP

Liquid