Periostin-antilichaam/POSTN

Periostin (PN), also designated osteoblast-specific factor 2 (OSF-2), is a disulfide linked protein originally isolated as a osteoblast-specific factor. Periostin is a secreted protein that binds heparin and functions as a ligand for AlphaVbeta3 and AlphaVbeta5 integrins. In preosteoblasts, Periostin acts as a cell adhesion molecule and plays a role in osteoblast recruitment, spreading and attachment. Periostin is mainly detected in lower gastrointestinal tract, aorta, stomach, placenta, uterus and breast tissues but is up-regulated in epithelial ovarian tumors and overexpressed in breast cancer. Expression of Periostin is increased by bone morphogenetic protein (BMP2) and transforming growth factor beta 1 (TGF beta 1) . Periostin contains a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain, which shows homology with the insect protein fasciclin, and a C-terminal domain.

Immunohistochemistry (FFPE) : 1-2 µg/mL

Q15063

Human

A portion of amino acids 193-326 from human POSTN protein was used as the immunogen for the Periostin antibody.

POSTN/4096

IHC-P

Protein G affinity

Purified

This Periostin antibody is available for research use only.

Aliquot the Periostin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

1 mg/mL in 1X PBS; BSA free, sodium azide free

Optimal dilution of the Periostin antibody should be determined by the researcher.

Secreted, Cytoplasm

IHC staining of FFPE human adrenal gland tissue with Periostin antibody (clone POSTN/4096) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.