PHKG2 (NM 000294) Humaan Recombinant eiwit

Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9C, also known as autosomal liver glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]

Purified recombinant protein of Human phosphorylase kinase, gamma 2 (testis) (PHKG2), transcript variant 1, Met1-Leu216, with N-terminal His tag, expressed in E. coli, 50ug

GSD9C

5261

P15735

NM_000294

16p11.2

E. coli

N-His

Calcium signaling pathway, Insulin signaling pathway

Calcium signaling pathway, Insulin signaling pathway

>50 ug/mL as determined by microplate BCA method

> 80% as determined by SDS-PAGE and Coomassie blue staining

Liquid

25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1% Sarkosyl.

Druggable Genome, Protein Kinase

24.7 kDa

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

PHKG2

Human

NP_000285

https://www.ncbi.nlm.nih.gov/gene/5261

https://www.uniprot.org/uniprot/P15735

https://www.ncbi.nlm.nih.gov/nuccore/NM_000294

https://www.ncbi.nlm.nih.gov/nuccore/NP_000285