PHPT1 cDNA

PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides.

CGI-202, HEL-S-132P, HSPC141, PHP14

9q34.3

Human

PATGen (puc19-derived cloning vector)

ATGGCGGTGG CGGACCTCGC TCTCATTCCT GATGTGGACA TCGACTCCGA CGGCGTCTTC AAGTATGTGC TGATCCGAGT CCACTCGGCT CCCCGCTCCG GGGCTCCGGC TGCAGAGAGC AAGGAGATCG TGCGCGGCTA CAAGTGGGCT GAGTACCATG CGGACATCTA CGACAAAGTG TCGGGCGACA TGCAGAAGCA AGGCTGCGAC TGTGAGTGTC TGGGCGGCGG GCGCATCTCC CACCAGAGTC AGGACAAGAA GATTCACGTG TACGGCTATT CCATGGCCTA TGGTCCTGCC CAGCACGCCA TTTCAACTGA GAAAATCAAA GCCAAGTACC CCGACTACGA GGTCACCTGG GCTAACGACG GCTACTGA

PHPT1, CGI-202, HEL-S-132P, HSPC141, PHP14, PHPT1, ATGD0096-10 µg, ATGD0096-20 µg, ATGD0096-50 µg, ATGD0096-100 µg, ATGD0096-250 µg, ATGD0096-500 µg, ATGD0096-1 mg, ATGD0096-10, ATGD0096-20, ATGD0096-50, ATGD0096-100, ATGD0096-250, ATGD0096-500, ATGD0096-1

Store the plasmid at -20C.

Lyophilized

Enzymes & Proteases

610167

NP_054891.2

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_014172.5

378bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.