Pim-1 (fosfo Tyr309) Antilichaam

Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3) . Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis.

5292

P06803

NP_001230115.1

Human, Mouse, Rat

Pim-1 (phospho Tyr309) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 309 (A-A-V (p) -W-S) derived from Human Pim-1.

Unconjugated

Primary Antibodies

Phospho-Specific

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Phospho-Specific

Blue Ice

Store antibody at -20˚ C for up to one year.

45 kDa

IgG

The antibody detects endogenous levels of Pim-1 only when phosphorylated at tyrosine 309.

Western Blot: 1:500~1:1000

PIM1

Pim-1 oncogene

Homo sapiens

Selten G., Cell 46:603-611 (1986) .

Saris C.J., EMBO J. 10:655-664 (1991) .

Maita H., Eur. J. Biochem. 267:5168-5178 (2000) .

PIM-1, Proto-oncogene serine/threonine-protein kinase pim-1, EC 2.7.11.1

WB

83305339

Liquid