PIN4 cDNA

PIN4, also known as Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4, is peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and is essential for cell cycle regulation. There are two different isoforms which are PAR14 and PAR17. PIN4/PAR17 was imported into mitochondria in a time and membrane potential-dependent manner, where it reached the mitochondrial matrix. Moreover, Pin4 protein was shown to bind to double-stranded DNA under physiological salt conditions

EPVH, PAR14

Human

PATGen (puc19-derived cloning vector)

ATGCCCATGG CGGGGCTTCT AAAGGGGCTT GTACGGCAAC TGGAGCAGTT CAGAGTTCAA CAACAAGCTT CCAAGATGCC GCCCAAAGGA AAAAGTGGTT CTGGAAAAGC GGGGAAAGGG GGAGCAGCCT CTGGGAGTGA CAGTGCTGAC AAGAAGGCTC AAGGTCCCAA AGGTGGTGGC AATGCAGTAA AGGTCAGACA CATTCTATGT GAAAAACATG GCAAAATCAT GGAAGCCATG GAAAAGTTAA AGTCTGGGAT GAGATTCAAT GAAGTGGCCG CACAGTATAG TGAAGATAAA GCCAGGCAAG GGGGTGACTT GGGTTGGATG ACCAGAGGGT CCATGGTGGG ACCATTTCAA GAAGCAGCAT TTGCCTTGCC TGTAAGTGGG ATGGATAAGC CTGTGTTTAC AGACCCACCG GTTAAGACAA AATTTGGATA TCATATTATT ATGGTCGAAG GAAGAAAATA A

PIN4, EPVH, PAR14, PIN4, ATGD0020-10 µg, ATGD0020-20 µg, ATGD0020-50 µg, ATGD0020-100 µg, ATGD0020-250 µg, ATGD0020-500 µg, ATGD0020-1 mg, ATGD0020-10, ATGD0020-20, ATGD0020-50, ATGD0020-100, ATGD0020-250, ATGD0020-500, ATGD0020-1

Store the plasmid at -20C.

Lyophilized

Metabolism

300252

AAH93700.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

BC093700.1

471bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.