PJA1 Antilichaam

Ubiquitinization is an important cellular degradation process requiring sequential reactions that are mediated by three enzymes: E1, E2 and E3. PJA1, also known as Praja1 and RING finger protein 70, is a 643 amino acid E2-dependent E3-ubiquitin ligase that is abundantly expressed in the brain (1,2) . Through interaction and activation with the E2-ubiquitin ligase UBC4, PJA1 mediates substrate-specific ubiquitination via its RING finger domain and facilitates ubiquitination (3) . Overexpression of PJA1 in gastrointestinal cancers suggests that it may be responsible for the degradation of some anti-oncogenic proteins (4,5) .

64219

Q8NG27

NP_660095

Human, Mouse, Rat

Unconjugated

Primary Antibodies

Cancer, Homeostasis

PJA1 antibody is affinity chromatography purified via peptide column.

Cat. No. 1303 - Human Brain Tissue Lysate

1 mg/mL

PJA1 antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

PJA1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

IgG

PJA1 antibody is human, mouse and rat reactive. At least two isoforms of PJA1 are known to exist; this antibody will detect both isoforms.

PJA1

Praja ring finger 1, E3 ubiquitin protein ligase

Homo sapiens

Yu P, Chen Y, Tagle DA, et al. PJA1, encoding a RING-H2 finger ubiquitin ligase, is a novel human X chromosome gene abundantly expressed in brain. Genomics 2002; 79:869-74.

Zoabi M, Sadeh R, de Bie P, et al. PRAJA1 is a ubiquitin ligase for the polycomb repressive complex 2 proteins. Biochem. Biophys. Res. Commun. 2011; 408:393-8.

Doyle JM, Gao J, Wang J, et al. MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases. Mol. Cell 2010; 39:963-74.

Saha T, Vardhini D, Tang Y, et al. RING finger-dependent ubiquitination by PRAJA is dependent on TGF-beta and potentially defines the functional status of the tumor suppressor ELF. Oncogene 2006; 25:693-705.

Praja ring finger 1, E3 ubiquitin-protein ligase Praja-1, PRAJA1, RING finger protein 70, RNF70

ELISA, WB, IHC-P, IF

41281725

Liquid